물리학과 특별세미나 개최 안내
물리학과에서는 특별세미나를 아래와 같이 개최하오니 관심있는 분들의 많은 참석을 바랍니다.
1. 연사: 강준호 박사 (KIST)
2. 일시: 2022. 11. 14(월), 오후 4시 30분
3. 장소: 공학3동 302호 (세미나실)
4. 제목: Physical dynamics of single 검증 바카라s
Physical properties of 검증 바카라s, such as mass, volume, and stiffness are valuable indicators of 검증 바카라 state and function. Numerous 검증 바카라ular processes including 검증 바카라 development, differentiation, migration, and disease progression (e.g., cancer metastasis) accompany changes in 검증 바카라ular physical properties. However, studying the physical dynamics is challenging because both the magnitude and duration of 검증 바카라ular changes are heterogeneous across the 검증 바카라 population. In this talk, we present new methods to monitor single-검증 바카라 stiffness, bioenergetics, and mass over the entire 검증 바카라 cycle with a temporal resolution of ~1 min.
We utilize the suspended microchannel resonator (SMR), a fluid-filled cantilever capable of measuring the buoyant mass of a 검증 바카라 by the change of SMR resonant frequency. First, we quantify 검증 바카라 stiffness via acoustic scattering of waves from a 검증 바카라 inside the SMR. Through simulations, experiments with hydrogels, and chemical perturbation of 검증 바카라s, we show that our readout from acoustic scattering measures stiffness1. Second, we monitor the mitochondrial membrane potential of single mouse lymphocytic leukemia L1210 검증 바카라s and model ATP synthesis dynamics using an electrical circuit model of mitochondria2. We found that mitochondrial ATP synthesis temporarily decreases by approximately 50% during early mitosis. Lastly, we monitored L1210 검증 바카라 growth during normal proliferation and polyploidization to show that increasing 검증 바카라 mass by over 100-fold does not limit maximal growth efficiency, indicating exponential growth3. Altogether, these studies revealed physical dynamics and their underlying mechanism throughout the 검증 바카라 cycle that have not been observed by endpoint assays.
[1] Kang, et al. “Non-invasive monitoring of single-검증 바카라 mechanics by acoustic scattering”. Nature Methods (2019).
[2] Kang*, Katsikis*, et al. “Monitoring and modeling of lymphocytic leukemia 검증 바카라 bioenergetics reveals decreased ATP synthesis during 검증 바카라 division”. Nature Communications (2020).
[3] Mu*, Kang*, et al. “Mass measurements during lymphocytic leukemia 검증 바카라 polyploidization decouple 검증 바카라 cycle-and 검증 바카라 size-dependent growth”. Proceedings of the National Academy of Sciences (2020).
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