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Toward an Unified Description for Thermodynamics,
    Folding Kinetics, and Mutagenesis of Proteins”

* Speaker : Prof. Ik Soo Chang[Pusan National University]
* Place : Physics Seminar Room (Science Bldg, 3-201)
* Date & Time : Oct,바카라 양방 배팅16Fri)바카라 양방 배팅4:00 ~ 5:00 pm
 
Abstract
A multiscale-heterogeneous protein energy function was developed for the biophysical study of the
thermodynamics, folding kinetics, and mutagenesis of proteins. After hybridizing an바카라 양방 배팅atomic바카라 양방 배팅force-
field into a coarse-grained energy function to account for the specificity of a바카라 양방 배팅sequence바카라 양방 배팅of바카라 양방 배팅amino
acids even with the high structural homology, we constructed an exact free energy landscape of a
given protein and characterized its thermodynamics and folding-unfolding kinetics via바카라 양방 배팅a바카라 양방 배팅Chevron
plot based on solving exactly a master equation, which would enable us to pursue바카라 양방 배팅a바카라 양방 배팅mutagenesis
application. Our approach was applied successfully for resolving a controversy바카라 양방 배팅about바카라 양방 배팅the바카라 양방 배팅folding
mechanism of fast folding proteins.  

Recent experiments claiming that BBL protein follows a global downhill folding raised an important
controversy as to the folding mechanism of fast folding proteins. Under the global downhill folding
scenario not only proteins undergo a gradual folding, but also folding events along the continuous
folding pathway could be mapped out from the equilibrium denaturation experiment. Based on the
exact calculation using a free energy landscape, relaxation eigenmodes from바카라 양방 배팅a바카라 양방 배팅master바카라 양방 배팅equation,
and Monte Carlo simulation of an바카라 양방 배팅extended바카라 양방 배팅Munoz-Eaton바카라 양방 배팅model바카라 양방 배팅that바카라 양방 배팅incorporates바카라 양방 배팅multiscale-
heterogeneous pairwise-interactions between amino acids, here we show that the very nature of a
two-state cooperative transition such as a bimodal distribution from an exact free energy landscape
and biphasic relaxation kinetics do manifest in the thermodynamics바카라 양방 배팅and바카라 양방 배팅folding-unfolding바카라 양방 배팅kinetics
of BBL and peripheral subunit-binding domain (PSBD) homologue. Our results provide an unequivocal
resolution to the fundamental controversy related to바카라 양방 배팅the바카라 양방 배팅global바카라 양방 배팅downhill바카라 양방 배팅folding바카라 양방 배팅scheme,바카라 양방 배팅whose
applicability to other proteins should be critically re-examined.

Contact Person : Prof. Ki Bong Lee (054-279-2068, kibong@postech.ac.kr)

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