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Toward an Unified Description for Thermodynamics,
    Folding Kinetics, and Mutagenesis of Proteins”

* Speaker : Prof. Ik Soo Chang[Pusan National University]
* Place : Physics Seminar Room (Science Bldg, 3-201)
* Date & Time : Oct,바카라 확률16Fri)바카라 확률4:00 ~ 5:00 pm
 
Abstract
A multiscale-heterogeneous protein energy function was developed for the biophysical study of the
thermodynamics, folding kinetics, and mutagenesis of proteins. After hybridizing an바카라 확률atomic바카라 확률force-
field into a coarse-grained energy function to account for the specificity of a바카라 확률sequence바카라 확률of바카라 확률amino
acids even with the high structural homology, we constructed an exact free energy landscape of a
given protein and characterized its thermodynamics and folding-unfolding kinetics via바카라 확률a바카라 확률Chevron
plot based on solving exactly a master equation, which would enable us to pursue바카라 확률a바카라 확률mutagenesis
application. Our approach was applied successfully for resolving a controversy바카라 확률about바카라 확률the바카라 확률folding
mechanism of fast folding proteins.  

Recent experiments claiming that BBL protein follows a global downhill folding raised an important
controversy as to the folding mechanism of fast folding proteins. Under the global downhill folding
scenario not only proteins undergo a gradual folding, but also folding events along the continuous
folding pathway could be mapped out from the equilibrium denaturation experiment. Based on the
exact calculation using a free energy landscape, relaxation eigenmodes from바카라 확률a바카라 확률master바카라 확률equation,
and Monte Carlo simulation of an바카라 확률extended바카라 확률Munoz-Eaton바카라 확률model바카라 확률that바카라 확률incorporates바카라 확률multiscale-
heterogeneous pairwise-interactions between amino acids, here we show that the very nature of a
two-state cooperative transition such as a bimodal distribution from an exact free energy landscape
and biphasic relaxation kinetics do manifest in the thermodynamics바카라 확률and바카라 확률folding-unfolding바카라 확률kinetics
of BBL and peripheral subunit-binding domain (PSBD) homologue. Our results provide an unequivocal
resolution to the fundamental controversy related to바카라 확률the바카라 확률global바카라 확률downhill바카라 확률folding바카라 확률scheme,바카라 확률whose
applicability to other proteins should be critically re-examined.

Contact Person : Prof. Ki Bong Lee (054-279-2068, kibong@postech.ac.kr)

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