Dynamic Force-Fluorescence Spectroscopy of a DNA 바카라 배팅 법 Junction Rev…

As mechanical parameters are being recognized as important determinants of many biological processes, single-molecule manipulation techniques have been continually evolving to break new records in their sensitivity and stability 1-3. However, one biologically relevant detection regime that has been out of reach is that of high spatial resolution under weak applied forces. We have therefore developed a new single-molecule technique, whereby mechanical manipulation via optical tweezers and observation of molecular motion via single-molecule FRET are combined without compromising their individual capabilities. Using this approach we have studied the conformational dynamics of the 바카라 배팅 법 junction under applied stretching force, that has revealed the existence a hidden intermediate state along the reaction coordinate. The length of helical arms is shown to be an important factor determining force sensitivity of the 바카라 배팅 법 junction. This suggests that the recombination process occurring in the cell could be extremely sensitive to sub-pico Newton forces that last only milliseconds.